3D Histological Reconstruction of Fiber Tracts and Direct Comparison with Diffusion Tensor MRI Tractography

Julien Dauguet, Sharon Peled, Vladimir Berezovskii, Thierry Delzescaux, Simon K. Warfield, Richard Born, C.-F. Westin
Ninth International Conference on Medical Image Computing and Computer-Assisted Intervention (MICCAI'06)
Pages 109-116
October, 2006

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Abstract

A classical neural tract tracer, WGA-HRP, was injected at multiple sites within the brain of a macaque monkey. Histological sections of the labeled fiber tracts were reconstructed in 3D, and the fibers were segmented and registered with the anatomical post-mortem MRI from the same animal. Fiber tracing along the same pathways was performed on the DTI data using a classical diffusion tracing technique. The fibers derived from the DTI were compared with those segmented from the histology in order to evaluate the performance of DTI fiber tracing.While there was generally good agreement between the two methods, our results reveal certain limitations of DTI tractography, particularly at regions of fiber tract crossing or bifurcation.

Three different views of the paths derived from histological slices (a, b, c), the corresponding fiber tracts derived from DTI (d, e, f), and both results displayed together (g, h, i)

Reference

Dauguet J, Peled S, Berezovskii V, Delzescaux T, Warfield SK, Born R, Westin CF. 3D histological reconstruction of fiber tracts and direct comparison with diffusion tensor MRI tractography. In Ninth International Conference on Medical Image Computing and Computer-Assisted Intervention (MICCAI'06), Lecture Notes in Computer Science 4190. Copenhagen, Denmark, 2006;109-116.

Bibtex entry

@InProceedings{dauguetMICCAI06,
  Author         = "Julien Dauguet and Sharon Peled and Vladimir Berezovskii   
                   and  Thierry Delzescaux and Simon K. Warfield and Richard   
                   Born  and Carl-Fredrik Westin",                             
  title          = "{3D} Histological Reconstruction of Fiber Tracts and Direct
                    Comparison with Diffusion Tensor {MRI} Tractography",      
  booktitle      = "Ninth International Conference on Medical Image Computing  
                   and Computer-Assisted Intervention (MICCAI'06)",            
  month          = "October",                                                  
  year           = "2006",                                                     
  pages          = "109--116",                                                 
  series         = "Lecture Notes in Computer Science 4190",                   
  address        = "Copenhagen, Denmark"}

Grants

NIH P41-RR13218 (NAC), NIH R01-MH50747, NIH P30-EY12196, NIH U41-RR019703, NIH U54-EB005149 (NAMIC), NSF ITR 0426558, NMSS RG-3478A2/2, NIH R21-MH67054

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